Screening of anxiolytic properties and analysis of structure-activity relationship of new derivatives of 6-(4-methoxy)-7H-[1,2,4]triazolo[3,4-a][2,3]benzodiazepine under the code RD

Introduction: Searching for new compounds with anti-anxiety activity resulting from the combination of privileged scaffolds is a promising direction in medicinal chemistry and development drugs. Anxiolytic potential cytotoxic properties previously synthesized molecules, containing fragments 2,3-benzodiazepine 1,2,4-triazole – 6-(4-methoxyphenyl)-7H-[1,2,4]triazolo[3,4-A][2,3]benzodiazepines under generic code RD were studied.
 Materials methods: Screening anxiolytic was performed on elevated plus maze (EPM) open field (OF) test models. Structural functional analysis studied substances carried out. A degree muscle relaxant effect assessed tests Grid, Wire, Rotarod. cytotoxicity study out using an MTT assay human hepatocellular carcinoma cells HepG2.
 Results discussion: For number novel triazolo[3,4-a][2,3]benzodiazepine derivatives, prominent manifested terms EPM test. The results OF consistent obtained data confirmed presence sought leading compounds. There no significant tone study. It observed that possessed safe further studies vivo.
 Conclusion: Among derivatives 6-(4-methoxyphenyl)-7H-[1,2,4]triazolo[3,4-a][2,3]benzodiazepine RD, (RD-4, 12, 13) high comparable to diazepam tofisopam found. most compound RD-4 due its pronounced low properties.